human interleukin 1β (Miltenyi Biotec)

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Miltenyi Biotec human interleukin 1β
Human Interleukin 1β, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+1%CE%B2/Human+IL-1%CE%B2%2C+premium+grade/pm42478839-454-20-22
Average 94 stars, based on 28 article reviews

human interleukin 1β - by Bioz Stars, 2026-09

94/100 stars

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Staining:

Article Title: Microbiome modulation uncouples efficacy and toxicity induced by immune checkpoint blockade in mouse multiple myeloma.
Article Snippet: Anti-human IFNγ (1μg/mL; Cat. 506532, Biolegend, Inc) and anti-human IL-4 (2μg/ml; Cat. 500838, Biolegend, Inc) were added to the culture to inhibit differentiation towards Th1 and Th2, respectively. .. Conditioning of mouse Th17 cells with SCFAs Mouse-derived splenocytes were stained with CellTraceTM Violet Cell Proliferation kit (Thermo Fisher) and cultured in vitro for 5 days with DynabeadsTM Mouse T-Activator CD3/CD28 (Cat. 11452D, ThermoFisher Scientific) under optimal Th17-polarizing conditions: TGF-β (2 ng/mL; Cat. 100-21 C, Peprotech, Inc.), IL-6 (20 ng/mL; Cat. 216-16, Peprotech, Inc.), IL-1β (10 ng/mL; Cat. 130-101-680, Miltenyi Biotech). anti-mouse IFNγ (10 μg/mL; Cat. 505834, Biolegend, Inc) and antimouse IL-4 (10 μg/mL; Cat. 504122, Biolegend, Inc) were added to the culture to inhibit differentiation towards Th1 and Th2, respectively. .. At day 0 sodium acetate or sodium butyrate (Sigma-Aldrich) were added to the medium at 1mMworking concentration andmaintained for the entire experiment.

Article Title: Microbiome modulation uncouples efficacy and toxicity induced by immune checkpoint blockade in mouse multiple myeloma
Article Snippet: Anti-human IFNγ (1 μg/mL; Cat. 506532, Biolegend, Inc) and anti-human IL-4 (2 μg/ml; Cat. 500838, Biolegend, Inc) were added to the culture to inhibit differentiation towards Th1 and Th2, respectively. .. Mouse-derived splenocytes were stained with CellTraceTM Violet Cell Proliferation kit (Thermo Fisher) and cultured in vitro for 5 days with DynabeadsTM Mouse T-Activator CD3/CD28 (Cat. 11452D, ThermoFisher Scientific) under optimal Th17-polarizing conditions: TGF-β (2 ng/mL; Cat. 100-21 C, Peprotech, Inc.), IL-6 (20 ng/mL; Cat. 216-16, Peprotech, Inc.), IL-1β (10 ng/mL; Cat. 130-101-680, Miltenyi Biotech). anti-mouse IFNγ (10 μg/mL; Cat. 505834, Biolegend, Inc) and anti-mouse IL-4 (10 μg/mL; Cat. 504122, Biolegend, Inc) were added to the culture to inhibit differentiation towards Th1 and Th2, respectively. .. At day 0 sodium acetate or sodium butyrate (Sigma-Aldrich) were added to the medium at 1 mM working concentration and maintained for the entire experiment.

Cell Culture:

In Vitro:

Incubation:

Article Title: Overcoming claudin family homology: discovery of ARC101, a highly potent CLDN6-specific T-cell engager with a novel CD3 binder for ovarian adenocarcinoma.
Article Snippet: Immature DCs were harvested by adding cold DPBS and incubating on ice for 15–30 min. Antigen stimulation and maturation: DCs were seeded at 2.0–3.0 × 106 cells/500 μL per well in 48-well plates and incubated for 4 h at 37°C with 0.6 μM ARC101, bevacizumab (MedChem, Cat# HYP9906), trastuzumab (MedChem, Cat# HY-P9907), 240 μg/mL keyhole limpet hemocyanin (KLH), or 2 μg/ mL CEFTA (Mabtech, Cat# 3617–1). .. DC maturation was then induced using a cocktail containing TNF-α (Miltenyi Biotec, Cat# 130–094-024) and IL-1β (Miltenyi Biotec, Cat# 130–093-898), followed by a 40–42 h incubation. .. Co-culture with CD4+ T cells: in parallel, CD4+ T cells were isolated from the same donor PBMCs using the Human CD4+ T Cell Isolation Kit (StemCell, Cat# 17952) and co-cultured with mature DCs at a 1:10 ratio (DC:T cell) for 6 days at 37°C.

Article Title: Overcoming claudin family homology: discovery of ARC101, a highly potent CLDN6-specific T-cell engager with a novel CD3 binder for ovarian adenocarcinoma
Article Snippet: Immature DCs were harvested by adding cold DPBS and incubating on ice for 15–30 min. Antigen stimulation and maturation: DCs were seeded at 2.0–3.0 × 10 6 cells/500 μL per well in 48-well plates and incubated for 4 h at 37°C with 0.6 μM ARC101, bevacizumab (MedChem, Cat# HY-P9906), trastuzumab (MedChem, Cat# HY-P9907), 240 μg/mL keyhole limpet hemocyanin (KLH), or 2 μg/mL CEFTA (Mabtech, Cat# 3617–1). .. DC maturation was then induced using a cocktail containing TNF-α (Miltenyi Biotec, Cat# 130–094-024) and IL-1β (Miltenyi Biotec, Cat# 130–093-898), followed by a 40–42 h incubation. .. Co-culture with CD4 + T cells: in parallel, CD4 + T cells were isolated from the same donor PBMCs using the Human CD4 + T Cell Isolation Kit (StemCell, Cat# 17952) and co-cultured with mature DCs at a 1:10 ratio (DC:T cell) for 6 days at 37°C.

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